导师姓名：吕正兵
性别：男
人气指数：454

所属院校：浙江理工大学
所属院系：生命科学学院
职称：教授
导师类型：
招生专业：生物学
研究领域： 1.海洋多肽类药物及其作用机制的研究；2.抗菌肽及兽用疫苗的开发利用。
研究领域： 1.海洋多肽类药物及其作用机制的研究；2.抗菌肽及兽用疫苗的开发利用。 [收起]




通讯方式 :
办公电话：**
通讯地址：杭州市下沙高教园2号928生命科学学院


个人简述 :
男，1969年10月生，安徽庐江人，1993年毕业于安徽师范大学生物系， 2004年6月获中国药科大学微生物与生化药学专业博士学位。现为浙江理工大学教授、生命科学院院党委委员、生物制药系主任、浙江省家蚕生物反应器和生物医药重点实验室副主任，浙江省生物化学与分子生物学学会副理事长，中国生物化学与分子生物学教学专业委员会委员，中国生物化学与分子生物学海洋专业委员会委员, 中华医学会航海医学分会第七届海洋生物工程委员会常务委员，中华医学会航海医学分会东海分会委员, 浙江省 “151人才”，曾为新加坡国立大学医学院高级访问学者，主持和参与863项目等国家级和省部级等项目20余项，发表学术论文100余篇，其中SCI 41篇，获授权发明专利10项，获省部级奖励4项，指导大学生挑战杯获国家二等奖1项，长期从事海洋多肽类药物和昆虫抗菌肽研究。


科研工作 :
发表主要论文和著作:
1. Chen J, Shu T, Chen J, Ye M, Lv Z, Nie Z, Gai Q, Yu W, Zhang Y. Purification and Initial Functions of Sex-Specific Storage Protein 2 in Bombyx mori Protein J, 2015, 34(4):256-66.

2. Nie Z, Zhu H, Zhou Y, Wu C, Liu Y, Sheng Q, Lv Z, Zhang W, Yu W, Jiang C, Xie L, Zhang Y, Yao J. Comprehensive profiling of lysine acetylation suggests the widespread function is regulated by protein acetylation in the silkworm, Bombyx mori. Proteomics, 2015, 15(18):3253-66.

3. Li Y, Wang W, Cheng D, Wang T, Lu C, Chen J, Nie Z, Zhang W, Lv Z, Wu W, Shu J. A New Member of the TBC1D15 Family from Chiloscyllium plagiosum: Rab GTPase-Activating Protein Based on Rab7 as a Substrate. Mar Drugs, 2015, 13(5):2955-66.

4. Zhang W, Wang J, Zhang C, Fang Q, Shu J, Li S, Jin J, Wang D, Nie Z, Lv Z, Zhang Y. Synergetic Protein Factors That Improve rhGM-CSF Absorption via an Oral Route Exist in Silkworm Pupae. Mol Pharm, 2015, 12(5):1347-55.

5. Li S, Wei Z, Chen J, Chen Y, Lv Z, Yu W, Meng Q, Jin Y. Oral administration of a fusion protein between the cholera toxin B subunit and the 42-amino acid isoform of amyloid-β peptide produced in silkworm pupae protects against Alzheimer's disease in mice. PLoS One, 2014, 9(12):e113585.

6. Chen J, Shu T, Lv Z, Nie Z, Chen J, Chen H, Yu W, Gai Q, Zhang Y. Purification and functional characterization of a protein: Bombyx mori human growth hormone like protein in silkworm pupa. PLoS One, 2014, 9(12):e114351.

7. Gong Y, Wang X, Indran IR, Zhang SJ, Lv Z, Li J, Holmes M, Tang YZ, Yong EL .Phytoplankton blooms: an overlooked marine source of natural endocrine disrupting chemicals. Ecotoxicol Environ Saf, 2014, 107:126-32.

8. Liu M, Wang C, Li D, Liu Y, Sheng Q, Lv Z, Yu W, Wang D, Zhang Y, Nie Z. Cloning and expression characteristics of the notch-associated gene BmE(spl)mγ from silkworm, Bombyx mori, Appl Biochem Biotechnol, 2014, 173(8):2065-75.

9.Liu Y, Gao Z, Guo Q, Wang T, Lu C, Chen Y, Sheng Q, Chen J, Nie Z, Zhang Y, Wu W, Lv Z and Shu J. Anti-Diabetic Effects of CTB-APSL Fusion Protein in Type 2 Diabetic Mice. Mar Drugs, 2014, 12(3), 1512-1529.

10. Lu C, Zhang J, Nie Z, Chen J, Zhang W, Ren X, Yu W, Liu L, Jiang C, Zhang Y, Guo J, Wu W, Shu J, and Lv Z. Study of MicroRNAs Related to the Liver Regeneration of the Whitespotted Bamboo Shark, Chiloscyllium plagiosum. BioMed Research International, 2013, ID 795676, 2013.

11. Zhang J , Liu Y, Zhang X, Nie Z, Chen J,Liu L, Li J, Zhang Y , Guo J, Lv Z . Identification of MicroRNAs in Chiloscyllium plagiosum (whitespotted bamboo shark) liver by Illumina sequencing. Gene, 2013,527（1）:259-65.

12. Liu Y, Chen Y, Chen J, Zhang W, Sheng Q, Chen J, Yu W, Nie Z, Zhang Y, Wu W, Lv Z. Expression of Active Peptide from Shark Liver Gene in Silkworm, Bombyx mori. and Preliminary Research on Oral Administration of the Recombinant Protein. Mar Drugs, 2013,11(5):1492-505.

13. Lv Z， Wang T, Zhuang W，Wang D， Chen J，Nie Z，Liu L，Zhang W，Wang L， Wang D，Wu X，Li J，Qian L， and Zhang Y. The Expression, Purification, and Characterization of a Ras Oncogene (Bras2) in Silkworm (Bombyx mori). International Journal of Genomics, 2013, Article ID 269609, 8 pages.

14. Lv Z, Liu Y, Chen Y, Li B, Liu L, Chen J, Nie Z, Sheng Q, Yu W, Zhang Y Expression and subcellular localization of a novel gene Bm-X of the silkworm, Bombyx mori. American Journal of Molecular Biology, 2012, 2, 224-231

15. Yang L, Lu X, Liu Y, Lv Z, Chen J, Yu W, Zhang Y, Nie Z. Expression analysis of miRNAs in BmN cells. Gene, 2012, 505(2):240-5.

16. Xu T, Wang B, Wang L, Zhang Y, Lv Z. Pinellia ternata agglutinin produced in Bombyx mori cells exhibits bioactivity. Acta Biochim Pol, 2012, 59(2):231-6. (Corresponding author)

17. Yu W, Li J, Wang M, Quan Y, Chen J, Nie Z, Lv Z, Zhang Y. The screening and functional study of proteins binding with the BmNPV polyhedrin promoter. Virol J, 2012, 9(1):90.

18. Quan Y, Liu G, Yu W, Nie Z, Chen J, Lv Z, Zhang Y. Expression, Purification, and Characterization of Ras Protein (BmRas1) from Bombyx mori. Comp Funct Genomics, 2012, 2012:747539.

19. Lv Z, Zhang X, Liu L, Chen J, Nie Z, Sheng Q, Zhang W, Jiang C, Yu W, Wang D, Wu X, Zhang S, Li J, Zhang Y. Characterization of a gene encoding prohibitin in silkworm, Bombyx mori. Gene. 2012, 502(2):118-24.

20. Zhang W, Zhang C, Lv Z, Fang D, Wang D, Nie Z, Yu W, Lan H, Jiang C, Zhang Y. Molecular characterization, tissue distribution, subcellular localization and actin-sequestering function of a thymosin protein from silkworm. PLoS One, 2012, 7(2):e31040.

21. Chen J, Gai Q, Lv Z, Chen J, Nie Z, Wu X,, Zhang Y. All-trans retinoic acid affects subcellular localization of a novel BmNIF3l pro,tein: functional deduce and tissue distribution of NIF3l gene from silkworm (Bombyx mori). Arch Insect Biochem Physiol, 2010, 74(4):217-31.

22. Lan H, Nie Z, Liu Y, Lv Z, Liu Y, Quan Y, Chen J, Zhen Q, Chen Q, Wang D, Sheng Q, Yu W, Chen J, Wu X, Zhang Y.In vivo bioassay of recombinant human growth hormone synthesized in B. mori pupae. J Biomed Biotechnol, 2010, 2010:306462.

23. Wu X, Yang D, Zhu X, Feng Z, Lv Z, Zhang Y, Shen B, Xu Z. Iso-migrastatin Titer Improvement in the Engineered Streptomyces lividans SB11002 Strain by Optimization of Fermentation Conditions. Biotechnol. Bioprocess Eng, 2010, 15(4):664-669.

24. Wang M, Cai J, Huang L, Lv Z, Zhang Y, Xu Z. High-level expression and efficient purification of bioactive swollenin in Aspergillus oryzae. Appl Biochem Biotechnol, 2010, 162(7):2027-36.

25. Zeng F, Xie H, Nie Z, Chen J, Lv Z, Chen J, Wang D, Liu L, Yu W, Sheng Q, Wu X, Zhang Y. Characterization of the gene BmEm4, a homologue of Drosophila E(spl)m4, from the silkworm, Bombyx mori. Comp Funct Genomics, 2009, 2009:947490.

26. Nie Z, Lv Z, Qian J, Chen J, Li S, Sheng Q, Wang D, Shen H, Yu W, Wu X, Zhang Y.Molecular cloning and expression characterization of translationally controlled tumor protein in silkworm pupae. Mol Biol Rep, 2010, 37(6):2621-8.

27. Lv Z, Ou Y, Li Q, Zhang W, Ye B, Wu W. Expression, purification and bioactivities analysis of recombinant active peptide from shark liver. Mar Drugs, 2009, 7(2):258-67.

28. Shen Y, Zhang T, Chen J, Lv Z, Chen J, Wang D, Nie Z, He P, Wang J, Zheng Q, Sheng Q, Wu X, Zhang Y. Molecular characterization and tissue localization of an F-box only protein from silkworm, Bombyx mori. Comp Funct Genomics, 2009, 2009:416040.

29. Zhang Y, Xia Q, Xu J, Chen J, Nie Z, Wang D, Zhang W, Chen J, Zheng Q, Chen Q, Kong L, Ren X, Wang J, Lv Z, Yu W, Jiang C, Liu L, Sheng Q, Jin Y, Wu X. Aligning the proteome and genome of the silkworm, Bombyx mori. Funct Integr Genomics, 2009, 9(4):447-54.

30. Zhang W, Lv Z, Nie Z, Chen G, Chen J, Sheng Q, Yu W, Jin Y, Wu X, Zhang Y. Bioavailability of orally administered rhGM-CSF: a single-dose, randomized, open-label, two-period crossover trial. PLoS One, 2009, 4(5):e5353. (并列第一）

31. Jin R, Lv Z, Chen Q, Quan Y, Zhang H, Li S, Chen G, Zheng Q, Jin L, Wu X, Chen J, Zhang Y. Safety and immunogenicity of H5N1 influenza vaccine based on baculovirus surface display system of Bombyx mori. PLoS One, 2008, 3(12):e3933.(并列第一）

32. Zhang Y(Corresponding author), Lv Z, Chen J, Chen Q, Quan Y, Kong L, Zhang H, Li S, Zheng Q, Chen J, Nie Z, Wang J, Jin Y, Wu X. A novel method for isolation of membrane proteins: a baculovirus surface display system.Proteomics, 2008, 8(20):4178-85.

33. Nie Z, Xu J, Chen J, Lv Z, Wang D, Sheng Q, Wu Y, Wang X, Wu X, Zhang Y. Expression analysis and characteristics of profilin gene from silkworm, Bombyx mori. Appl Biochem Biotechnol, 2009, 158(1):59-71.

34. Nie Z, Zhou R, Chen J, Wang D, Lv Z, He P, Wang X, Shen H, Wu X, Zhang Y. Subcellular localization and RNA interference of an RNA methyltransferase gene from silkworm, Bombyx mori. Comp Funct Genomics, 2008, 2008:571023.

35. He PA, Nie Z, Chen J, Chen J, Lv Z, Sheng Q, Zhou S, Gao X, Kong L, Wu X, Jin Y, Zhang Y. Identification and characteristics of microRNAs from Bombyx mori. BMC Genomics, 2008, 9:248.

36. Chen J, Chen J, Gai Q, Lv Z, Wang D, Nie Z, Wang J, Wang X, Wu X, Zhang Y. Molecular characterization and immunohis,tochemical localization of a novel troponin C during silkworm development.Cell Tissue Res, 2008, 331(3):725-38.

37. Kong L, Lv Z, Chen J, Nie Z, Wang D, Shen H, Wang X, Wu X, Zhang Y. Expression analysis and tissue distribution of two 14-3-3 proteins in silkworm (Bombyx mori). Biochim Biophys Acta, 2007, 1770(12):1598-604.

38. Zhang Y, Chen J, Lv Z, Nie Z, Zhang X, Wu X. Can 29kDa rhGM-CSF expressed by silkworm pupae bioreactor bring into effect as active cytokine through orally administration? Eur J Pharm Sci, 2006, 28(3):212-23.

39. Nie Z, Zhang Z, Wang D, He P, Jiang C, Song L, Chen F, Xu J, Yang L, Yu L, Chen J, Lv Z, Lu J, Wu X, Zhang Y. Complete sequence and organization of Antheraea pernyi nucleopolyhedrovirus, a dr-rich baculovirus. BMC Genomics. 2007, 8:248.

40. Wang X, Chen J, Lv Z, Nie Z, Wang D, Shen H, Wang X, Wu X, Zhang Y. Expression and functional analysis of the cellular retinoic acid binding protein from silkworm pupae (Bombyx mori). J Cell Biochem. 2007, 102(4):970-9.

41. Zheng Q, Chen J, Nie Z, Lv Z, Wang D, Zhang Y. Expression, purification and characterization of a three-domain Kazal-type inhibitor from silkworm pupae (Bombyx mori). Comp Biochem Physiol B Biochem Mol Biol. 2007, 146(2):234-40.

42. Huang F, Lv Z, Li Q, Wei L, Zhang L, Wu W. Study on hepatoprotective effect of peptide S-8300 from shark liver. World J Gastroenterol, 2005, 11(12):1809-12.

43.吕正兵 主编. 生物工程制药学，北京，2012，科学出版社。
承担主要项目

1. 主持完成浙江省自然科学基金项目，鲨肝活性肽S-12000抗糖尿病研究；

2. 主持完成浙江省科技厅项目，马传染性贫血病毒减毒中的膜蛋白基因突变研究；

3. 主持完成横向课题“白藜芦醇体外生物合成研究”；

4. 主持横向重大项目“猪繁殖与呼吸综合征病毒基因工程疫苗的开发研究”；

5. 主持横向重大项目“昆虫抗菌肽生物合成及规模化制备研究”；

6. 主持横项目“重组大流行流感灭活疫苗（家蚕杆状病毒）样品试制及工艺探索研究”；

7. 校内主持国家重大新药创制项目子课题“新型Ⅰ型糖尿病口服药物的研制和中试“

8. 参与完成国家“863”重点项目课题，家蚕生物反应器蛋白质药物规模化制备技术，主持经费409万；

9. 参与完成国家科技支撑计划，家蚕生物反应器生产新型人用禽流感疫苗的试制；

10.参与完成“973”项目子课题，家蚕小分子mRNA、小肽功能以及病原体与宿主相互作用机理研等。
我的育人成果

指导本科生获得多项省级和国家级学生科研奖项、校优秀毕业生等荣誉称号；

近5年指导的本科生导师制学生有3人分别被免推到北京师范大学、西南大学和复旦大学攻读硕士研究生；

指导研究生中有获得“理工十大成才先锋”、国家奖学金等荣誉称号；

曾获浙江省大学生“挑战杯”优秀指导教师称号；

多名学生硕士期间发表多篇第一作者SCI论文；

部分学生已在国外著名高校工作等。