导师姓名：刘逸寒
性别：男
人气指数：44

所属院校：天津科技大学
所属院系：生物工程学院
职称：副教授
导师类型：
招生专业：药理学
研究领域： [1]酶制剂开发与应用：酶分子的挖掘与进化，酶基因高效表达系统的构建与优化，酶蛋白发酵工艺... [展开]


研究领域： [1]酶制剂开发与应用：酶分子的挖掘与进化，酶基因高效表达系统的构建与优化，酶蛋白发酵工艺的建立与 [收起]




通讯方式 :
办公电话：**
电子邮件：lyh@tust.edu.cn
通讯地址：天津经济技术开发区第十三大街29号天津科技大学生物工程学院


个人简述 :
代表性专利：[1] 刘逸寒，路福平，樊帅，徐艳静，胡博，王春霞，王建玲．一种耐酸性高温α-淀粉酶及其基因工程菌和制备方法，ZL**9.6．[2] 刘逸寒，王正祥，路福平，王春霞，王建玲．一种耐高温α-淀粉酶及其制备方法和应用，ZL**8.3．[3] 刘逸寒，路福平，薄嘉鑫，王春霞，王建玲．一种细胞表面展示磷脂酶D酵母全细胞催化剂催化制备磷脂酰丝氨酸的方法，ZL**9.6．[4] 路福平，刘逸寒，张涛，刘晓光，王正祥，王春霞，王建玲．一种磷脂酶A2突变体及其制备方法，ZL**6.3．[5] 路福平，刘逸寒，张涛，刘晓光，王正祥，王春霞，王建玲．sn-2位为二十二碳六烯酸的磷脂酰丝氨酸的制备方法，ZL**7.8．[6] 路福平，刘逸寒，刘敏尧，刘靓，樊帅，王春霞，王建玲．碱性蛋白酶高产菌株及其所产的碱性蛋白酶，ZL**1.4．[7] 路福平，刘逸寒，郑宏臣，樊帅，王春霞 王建玲．一种耐高温高碱木聚糖酶及其基因工程菌和制备方法，ZL**4.9．[8] 路福平，刘逸寒，蔺松，王春霞，王建玲．一种抑制枯草芽孢杆菌谷氨酰胺转胺酶活性的多肽及其筛选方法和应用，ZL**8.7．[9] 路福平，刘逸寒，郑宏臣，王春霞，王建玲．一种耐高温木聚糖酶的高产菌株及利用该菌发酵产耐高温木聚糖酶的方法及得到的酶，ZL**9.X．[10] 李玉，荆玮，刘逸寒，刘晓光，王稳航，路福平．一种β-葡萄糖苷酶产生菌及利用该菌转化制备京尼平的方法，ZL**4.2．[11] 路福平，刘逸寒，刘敏尧，刘靓，薄嘉鑫，王春霞，王建玲．一种低温碱性蛋白酶及其制备方法，ZL**6.3．[12] 路福平，刘逸寒，薄嘉鑫，王春霞，王建玲．高活力磷脂酶D及细胞表面展示磷脂酶D酵母全细胞催化剂的制备，ZL**1.8．[13] 路福平，郝育杰，刘逸寒，王春霞，肖静，周浩，蒋彦洁，王珊．一种碱性果胶酶基因工程菌培养条件的优化方法，ZL**7.X[14] 路福平，杜连祥，刘逸寒，李玉，王建玲，王春霞．高温耐酸性α-淀粉酶的突变株及其构建方法，ZL**5.9．[15] 路福平，杜连祥，李涛，史文玉，刘逸寒．采用无机膜实现微生物原位分离发酵的方法，ZL**8.4．获奖情况[1] 一种耐酸性高温α-淀粉酶及其基因工程菌和制备方法．2017年度天津市专利奖金奖，排名第1．[2] 新型半纤维素酶创制关键技术的开发与应用．2016年度中国轻工业联合会科学技术进步奖二等奖，排名第1．[3] 耐高温α-淀粉酶的定向进化及其耐酸突变体在枯草芽孢杆菌中的表达．2013年度天津市优秀博士学位论文．[4] 重大淀粉酶品的创制绿色制造及其应用．2013年度国家技术发明奖二等奖，排名第6．[5] 一种耐高温高碱木聚糖酶及其基因工程菌和制备方法．2016年度天津市专利奖优秀奖，排名第2．[6] 高温耐酸性α-淀粉酶的突变株及其构建方法．2015年度天津市专利奖优秀奖，排名第3．[7] 果醋酿造的关键技术及产业化．2014年度中国商业联合会全国商业科技进步奖，排名第5．[8] 高产脂肪酶菌株的选育及发酵工艺优化．2014年度临沂市科技进步奖二等奖，排名第2．

科研工作 :
以第一作者先后发表各类科技论文30余篇，其中SCI收录22篇EI收录1篇，获得国家授权发明专利15项。代表性学术论文：[1]Liu YH* Li MJ Huang L Gui S Jia LB Zheng D Fu Y Zhang YT Rui JQ Lu FP*. Cloning expression and characterisation of phospholipase B from Saccharomyces cerevisiaeand its application in the synthesis of l-alpha-glyceryl phosphorylcholine and peanut oil degumming[J]. Biotechnology & Biotechnological Equipment doi.org/10.1080/**.2018.**.[2]Liu YH* Liu H Huang L Gui S Zheng D Jia LB Fu Y Lu FP*. Improvement in thermostability of an alkaline lipase I fromPenicillium cyclopiumby directed evolution[J]. RSC Advances 2017 7:38538-38548.[3] Liu YH* Huang L Jia LB Gui S Fu Y Zheng D Guo W Lu FP*. Improvement of the acid stability of Bacillus licheniformisalpha amylase by site-directed mutagenesis[J]. Process Biochemistry 2017 58:174-180.[4]Liu YH Huang L Guo W Jia LB Fu Y Gui S Lu FP*. Cloning expression and characterization of a thermostable and pH-stable laccase fromKlebsiella pneumoniaeand its application to dye decolorization[J]. Process Biochemistry 2017 53:125-134.[5] Liu YH Huang L Li MJ Liu H Guo W Gui S Niu JL Lu FP*. Characterization of the recombinant porcine pancreas phospholipase A2expressed in Pichia pastorisGS115 and its application to synthesis of 2-DHA-PS[J]. Process Biochemistry 2016 51(10):1472-1478.[6] Huang L1 Liu YH1 Liu XG Ban LT Wang Y Liu MJ Lu FP*. Functional expression of Trametes versicolor thermotolerant laccase variant in Pichia pastoris[J]. Biotechnology & Biotechnological Equipment 2016 30(2):261-269. (Co-first author)[7] Liu YH Huang L Liu WG Guo W Zheng HC WangJL Lu FP*. Studies on properties of the xylanbinding domain and linker sequence of xylanase XynG1-1 from Paenibacillus campinasensisG1-1[J]. Journal of Industrial Microbiology & Biotechnology 2015 42(12):1591-1599.[8]Liu YH Zhang T Qiao J Liu XG Bo JX Wang JL Lu FP*. High-yield phosphatidylserine production via yeast surface display of phospholipase D from Streptomyces chromofuscuson Pichia pastoris[J]. Journal of Agricultural and Food Chemistry 2014 62(23):5354-5360.[9]Liu YH Lin S Zhang XQ Liu XG Wang JL Lu FP*. A novel approach for improving the yield of Bacillus subtilistransglutaminase in heterologous strains[J]. Journal of Industrial Microbiology & Biotechnology 2014 41(8):1227-1235.[10]Liu YH Zhang T Zhang ZM Sun TY Wang JL Lu FP*. Improvement of cold adaptation of Bacillus alcalophilusalkaline protease by directed evolution[J]. Journal of Molecular Catalysis B: Enzymatic 2014 106:117-123.[11]Liu YH Fan S Liu XG Zhang ZM Wang JL Wang ZX* Lu FP*. A highly active alpha amylase from Bacillus licheniformis: directed evolution enzyme characterization and structural analysis[J]. Journal of Microbiology and Biotechnology 2014 24(7):898-904.[12]Liu YH Lin S Liu K Liu XG Zhang XQ Wang HB Lu FP*. High-level expression of the Streptomyces mobaraenseCICC11018 transglutaminase in Corynebacterium glutamicum ATCC13032[J]. Applied Biochemistry and Microbiology 2014 50(5):456-462.[13]Zheng HC1 Liu YH1 Sun MZ Han Y Wang JL Sun JS Lu FP*. Improvement of alkali stability and thermostability of Paenibacillus campinasensisFamily-11 xylanase by directed evolution and site-directed mutagenesis[J]. Journal of Industrial Microbiology & Biotechnology 2014 41(1):153-162. (Co-first author)[14]Wang YP1 Liu YH1 Wang ZX Lu FP*. Influence of promoter and signal peptide on the expression of pullulanase in Bacillus subtilis[J]. Biotechnology Letters 2014 36(9):1783-1789. (Co-first author)[15]Zhang Y1 Liu YH1 Li Y Liu XG Lu FP*. Extracellular expression of pullulanase from Bacillus naganoensisin Escherichia coli[J]. Annals of Microbiology 2013 63(1):289-294. (Co-first author)[16]Liu YH Hu B Xu YJ Bo JX Fan S Wang JL Lu FP*. Improvement of the acid stability of Bacillus licheniformisalpha amylase by error-prone PCR[J]. Journal of Applied Microbiology 2012 113(3):541-549.[17]Liu YH Chen GQ Wang JL Hao YJ Li M Li Y Hu B Lu FP*. Efficient expression of an alkaline pectate lyase gene from Bacillus subtilisand the characterization of the recombinant protein[J]. Biotechnology Letters 2012 34(1):109-115.[18]Zheng HC1 Liu YH1 Liu XG Han Y Wang JL Lu FP*. Overexpression of a Paenibacillus campinasensisxylanase in Bacillus megateriumand its applications to biobleaching of cotton stalk pulp and saccharification of recycled paper sludge[J]. Bioresource Technology 2012 125:182-187. (Co-first author)[19]Zheng HC1 Liu YH1 Liu XG Wang JL Han Y Lu FP*. Isolation purification and characterization of a thermostable Xylanase from a novel Strain Paenibacillus campinasensis G1-1[J]. Journal of Microbiology and Biotechnology 2012 22(7):930-938. (Co-first author)[20]Liu YH Lu FP* Chen GQ Snyder CL Sun J Li Y Wang JL Xiao J. High-level expression purification and characterization of a recombinant medium-temperature α-amylase from Bacillus subtilis[J]. Biotechnology Letters 2010 32(1):119-124.[21]Liu YH Lu FP* Li Y Yin XB Wang Y Gao C. Characterisation of mutagenised acid-resistant alpha-amylase expressed in Bacillus subtilisWB600[J]. Applied microbiology and biotechnology 2008 78(1):85-94. [22]Liu YH Lu FP* Li Y Wang JL Gao C. Acid stabilization of Bacillus licheniformisalpha amylase through introduction of mutations[J]. Applied microbiology and biotechnology 2008 80(5):795-803.[23]Liu YH Xu YJ Fan S BO JX Wang JL Lu FP*. Study on the influencing conditions in the electro-transformation efficiency of Bacillus licheniformisATCC14580[J]. Lecture Notes in Electrical Engineering 2014 251(3):1845-1853.