导师姓名：饶志明
性别：男
人气指数：677

所属院校：江南大学
所属院系：
职称：博士、教授、博士生导师
导师类型：
招生专业：
研究领域： 以国家需求为导向，结合采用传统微生物育种、基因组、蛋白质组、代谢组等现代组学技术改造、开发... [展开]


研究领域： 以国家需求为导向，结合采用传统微生物育种、基因组、蛋白质组、代谢组等现代组学技术改造、开发和应用微生物，积极开展工业生物技术创新并努力探索产业化道路，为人类服务。主要研究方向包括：1、高产氨基酸、维生素微生物代谢工程改造及产品开发2、生物基平台化合物的微生物高效制造3、高附加值药物中间体甾体激素生物转化关键技术研究及开发4、高产新型酶制剂基因工程菌的构建及其开发利用 [收起]




通讯方式 :
办公电话：**
电子邮件：raozhm@jiangnan.edu.cn


科研工作 :
【主要论著】（著作和论文）近几年在MetabolicEngineering,GreenChemistry,AminoAcids,JournalofSteroidBiochemistryandMolecularBiology,PlosOne,AppliedMicrobiologyandBiotechnology,JIndMicrobiolBiotechnol,ProcessBiochemistry,JournalofAppliedMicrobiology,JournalofBiotechnology,《化工学报》、《生物工程学报》、《微生物学报》等SCI、EI及核心期刊合作发表研究论文150多篇，其中SCI收录40多篇，获优秀论文奖2次。其中部分代表性研究论文如下:1.XianZhang,RongzhenZhang,TengBao,ZhimingRao*,TaoweiYang,MeijuanXu,ZhenghongXu,HuazhongLi*,ShangtianYang.Therebalancedpathwaysignificantlyenhancesacetoinproductionbydisruptionofacetoinreductasegeneandmoderate-expressionofanewwater-formingNADHoxidaseinBacillussubtilis.MetabolicEngineering.2014,23:34-41(SCI，IF8.26)2.RongzhenZhang,TaoweiYang,ZhimingRao*,HongmeiSun,MeijuanXu,XianZhang,ZhenghongXu,ShangtianYang.Efficientone-stepPreparationof&gamma-AminobutyricAcidfromGlucosewithoutExogenousCofactorbytheDesignedCorynebacteriumglutamicum.GreenChemistry.2014,16,4190-4197(SCI，IF6.83)3.MeijuanXu,ZhimingRao*,WenfangDou,JuanYang,JianJin,ZhenghongXu*.Site-directedmutagenesisandfeedback-resistantN-acetyl-L-glutamatekinase(NAGK)increaseCorynebacteriumcrenatumL-arginineproduction.AminoAcids,2012,43(1):255-266(SCI，IF4.1)4.WQZhang,MLShao,ZMRao*,MJXu,XZhang,TWYang,HLi,ZHXu*.Bioconversionof4-androstene-3,17-dionetoandrost-1,4-diene-3,17-dionebyrecombinantBacillussubtilisexpressingksddgeneencoding3-ketosteroid-△1-dehydrogenasefromMycobacteriumneoaurumJC-12.JournalofSteroidBiochemistryandMolecularBiology.2013,135:36-42(SCI，IF3.98.)5.YangTW,RaoZM*,ZhangX,XuMJ,XuZH*,YangST.Improvedproductionof2,3-butanediolinBacillusamyloliquefaciensbyover-expressionofglyceraldehyde-3-phosphatedehydrogenaseand2,3-butanedioldehydrogenase.PlosOne,2013,8(10):e76149(SCI，IF3.73)6.XianZhang,TengBao,ZhimingRao*,TaoweiYang,ZhenghongXu,ShangtianYang,HuazhongLi*.Two-stagepHControlStrategyBasedonThepHPreferenceofAcetoinReductaseRegulatesAcetoinand2,3-butanediolDistributioninBacillussubtilis.PlosOne,2014,9(3):e91187.(SCI，IF3.73)7.TengBao,XianZhang,ZhimingRao*,XiaojingZhao,RongzhenZhang,TaoweiYang,ZhenghongXu,ShangtianYang.Efficientwhole-cellbiocatalystforacetoinproductionwithNAD+regenerationsystemthroughhomologousco-expressionof2,3-butanedioldehydrogenaseandNADHoxidaseinengineeredBacillussubtilis.2014,9(7):e102951.(SCI，IF3.73)8.Tao-WeiYang,Zhi-MingRao*,XianZhang,Mei-JuanXu,Zheng-HongXu*,Shang-TianYang,Fermentationofbiodiesel-derivedglycerolbyBacillusamyloliquefaciens:E?ectsofco-substrateson2,3-butanediolproduction,AppliedMicrobiologyandBiotechnology.2013,97:7651-7658(SCI，IF3.69)9.ZhimingRao*,ZhengMa,WeiShen,HuiyingFang,JianZhugeandXiaoyuanWang.EngineeredSaccharomycescerevisiaeThatProduces1,3-PropanediolfromD-glucose.JournalofAppliedMicrobiology,2008，105：1768-1776（SCI，IF=2.6）10.RaoZhiming*,MaZheng,ShenWei,FangHuiying,ZhugeJian.Agrobacteriumtumefaciens-mediatedtransformationofindustrializedstrainCandidaglycerinogenesusingScGPD1genefromSaccharomycescerevisiae。JournalofBiotechnology，2008，36：313-317（SCI，IF=3.0）11.RaoZhiming*,MaZheng,ShenWei,FangHuiying,ZhugeJian.TransformationofindustrializedstrainCandidaglycerinogeneswithresistantgenezeocinviaAgrobacteriumtumefaciens.CurrentMicrobiology,2008,57(1):12-17（SCI，IF=1.5）12.MingmeiJia,MeijuanXu,BeibeiHe,ZhimingRao*.Cloning,expressionandcharacterizationofL-asparaginasefromanewlyisolatedBacillussubtilisB11-06.JAGRFOODCHEM.2013,2013,61(39):9428-9434.(SCI，IF2.9)13.MeijuanXu,ZhimingRao*,JuanYang,HaifengXia,WenfangDou,JianJin,ZhenghongXu*.HeterologousandhomologousexpressionoftheargininebiosyntheticargC~HclusterfromCorynebacteriumcrenatumforimprovementofL-arginineproduction.JIndMicrobiolBiotechnol.2012,39:495-502.(SCI，IF2.7)14.YangTW,RaoZM*,ZhangX,XuMJ,XuZH*,YangST.Effectsofcornsteepliquoronproductionof2,3-butanediolandacetoinbyBacillussubtilis.ProcessBiochemistry,2013,48:1610-1617(SCI，IF2.7)15.MeijuanXu,RongzhenZhang*,XiangyuLiu,JingsongShi,ZhenghongXu,ZhimingRao*,Improvingtheacidicstabilityof&beta-mannanasefromBacillussubtilisbysite-directedmutagenesis.ProcessBiochemistry.2013,48:1166-1173(SCI，IF2.7)16.XianZhang,RongzhenZhang*,TengBao,TaoweiYang,MeijuanXu,HuazhongLi,ZhenghongXu,ZhimingRao*,Moderate-expressionofthetranscriptionalregulatorALsRenhancesacetoinproductionbyBacillussubtilis.JIndMicrobiolBiotechnol.2013,40:1067-1076.(SCI，IF2.7)17.MinglongShao,ZhimingRao*,XianZhang,MeijuanXu,TaoweiYang,HuiLi,ZhenghongXu,ShangtianYang.Bioconversionofcholesterolto4-cholesten-3-onebyrecombinantBacillussubtilisexpressingchoMgeneencodingcholesteroloxidasefromMycobacteriumneoaurumJC-12.JournalofChemicalTechnology&Biotechnology.2014(SCI，IF2.5)18.JingGuo,ZhimingRao*,TaoweiYang,ZaiweiMan,MeijuanXu,XianZhang.High-levelproductionofmelaninbyanovelisolateofStreptomyceskathirae.FEMSMICROBIOLLETT.2014(SCI,2.0)19.MeijuanXu,ZhimingRao*,WenfangDou,ZhenghongXu*.TheRoleofARGRRepressorRegulationonL-arginineProductioninCorynebacteriumcrenatum.ApplBiochemBiotechnol.2013,170:587-597(SCI,IF=2.0)20.MeijuanXu,ZhimingRao*,HongXu,WenfangDou,XiaomeiZhang,HongyuXu,JianJin,ZhenghongXu*.EnhancedProductionofL-ArgininebyExpressionofVitreoscillaHemoglobinUsingaNovelExpressionSysteminCorynebacteriumcrenatum.ApplBiochemBiotechnol,2011,163(6):707-719.(SCI,IF=2.0)21.MeijuanXu,ZhimingRao*,JuanYang,WenfangDou,ZhenghongXu*.TheEffectofaLYSEExporterOverexpressiononl-ArginineProductioninCorynebacteriumcrenatum.CurrMicrobiol.2013,67:271-278(SCI，IF1.5)22.MeijuanXu,ZhimingRao*,WenfangDou,JianJin,ZhenghongXu*.Site-directedmutagenesisstudiesontheL-argininebindingsitesoffeedbackinhibitioninN-acetyl-L-glutamatekinase(NAGK)fromCorynebacteriumglutamicum.CurrMicrobiol,(2012)64:164-172..(SCI，IF1.5)23.TaoweiYang,ZhimingRao*,XianZhang,QingLin,HaifengXia,ZhenghongXu,ShangtianYang.Productionof2,3-butanediolfromglucosebyGRASmicroorganismBacillusamyloliquefaciens.JournalofBasicMicrobiology,2011.51,1&ndash9(SCI,IF1.5)24.XianZhang,TaoweiYang,JingZhang,MeijuanXu,HuazhongLi,ZhenghongXu&ZhimingRao*.MutationbreedingofacetoinhighproducingBacillussubtilisblockedin2,3-butanedioldehydrogenase.WorldJMicrobiolBiotechnol.2013,29:1783-1789(SCI,IF1.5)25.XianZhang,TaoweiYang,QingLin,MeijuanXu,ZhenghongXu,HuazhongLi,ZhimingRao*.Isolationandidentificationofanacetoinhighproductionbacteriumthatcanreversetransform2,3-butanedioltoacetoinatthedeclinephaseoffermentation.WorldJMicrobiolBiotechnol.(2011)27:2785&ndash2790.(SCI,IF1.5)26.TaoweiYang,XianZhang,ZhimingRao*,ShenghuiGu,HaifengXia,ZhenghongXu.Optimizationandscale-upof2,3-butanediolproductionbyBacillusamyloliquefaciensB10-127.WorldJMicrobiolBiotechnol.2012,28(4):1563-1574(SCI,IF1.5)27.Zai-weiMan,Zhi-mingRao*,Tao-weiYang,XianZhang,Mei-juanXu,Zheng-hongXu.EnhancedriboflavinproductionbyrecombinantBacillussubtilisRF1throughtheoptimizationofagitationspeed.WorldJMicrobiolBiotechnol.2013,accepted.28.ZhengMa,ZhimingRao*,BinZhuge,HuiyingFang,XiangruLiaoandJianZhuge*,ConstructionofaNovelExpressionSysteminKlebsiellapneumoniaeanditsApplicationfor1,3-PropanediolProduction.ApplBiochemBiotechnol**(2):399-407(SCIIF=2.0)29.ZhengMa,ZhimingRao*,LiyuXu,BinZhuge,HuiyingFang,XiangruLiaoandJianZhuge*,Expressionofdhaoperonrequiredfor1,3-PDformationinE.coliandSaccharomycescerevisiae.CurrentMicrobiology.201060(3):191-8(SCIIF=1.5)30.ZhengMa,ZhimingRao*,LiyuXu,BinZhuge,HuiyingFang,XiangruLiaoandJianZhuge*,Productionof1,3-propanediolfromglycerolbyengineeredEscherichiacoliusinganovelco-expressionvector.AfricanJournalofBiotechnologyVol.8(20),pp.5500-5505,19October,2009(SCI)31.XianzhongChen,HuiyingFang,ZhimingRao,etal.CloningandcharacterizationofanNAD+-dependentglycerol3-phosphatedehydrogenasegenefromCandidaglycerinogenes,anindustrialglycerolproducer.FEMSYeastResearch，2008,30(4):508-14（SCI，IF=2.2）32.XianzhongChen,HuiyingFang,ZhimingRao,WeiShen,BinZhuge,ZhengxiangWangandJianZhuge，AnefficientgenetictransformationmethodforglycerolproducerCandidaglycerinogenes,MicrobiologicalResearch,2008,163(5):531-537（SCI，IF=2.5）33.Fu-ZhuanWang,WeiShen,Zhi-MingRao,Hui-YingFang,Xiao-BeiZhanandJianZhuge.Constructionofaflocculatingyeastforfuelethanolproduction.BiotechnologyLetters,2008,30(1):97-102（SCI，IF=1.6）34.ZhangYongguang,ShenWei,RaoZhiming,FangHuiying,ZhugeJian.DeletionoftheCgTPIgeneencodingtriosephosphateisomeraseofCandidaglycerinogenesisinhibitsthebiosynthesisofglycerol,CurrentMicrobiology.2007,55(2):147-151.（SCI，IF=1.5）35.Shen,WeiWang,Zheng-XiangRao,Zhi-MingZhuge,JianZhuge,Bin.Agenetictransformationsystembasedontrp1complementationinCandidaglycerinogenes.WorldJournalofMicrobiologyandBiotechnology,2011,27(4)1005-1008（SCI，IF=1.5）36.BinZhuge,HuiFang,ZhimingRao,WeiShen,JianSong,JianZhuge.BioconversionoflovastatintoanovelstatinbyAmycolatopsissp.ApplMicrobiolBiotechnol.2008,79:209-216（SCI，IF=3.5）37.Zhang,XiaomeiLi,YinZhuge,BinShen,WeiRao,ZhimingFang,HuiyingZhuge,Jian.Optimizationof1,3-propanediolproductionbynovelrecombinantEscherichiacoliusingresponsesurfacemethodology.JournalofChemicalTechnologyandBiotechnology,2006,81(6):1075-1078.（SCI，IF=2.6）38.Zhang,XiaomeiLi,YinZhuge,BinShen,WeiRao,ZhimingFang,HuiyingZhuge,Jian.ConstructionofanovelrecombinantEscherichiacolsstraincapableofproducing1,3-propanediolandoptimizationoffermentationparametersbystatisticaldesign.WorldJournalofMicrobiology&Biotechnology,2006,22:945-952（SCI，IF=1.5）39.LiY,ShenW,WangZ,LiuJQ,RaoZ,TangX,FangH,ZhugeJ.IsolationandsequenceanalysisofthegeneURA3encodingtheorotidine-5'-phosphatedecarboxylasefromCandidaglycerinogenesWL2002-5,anindustrialglycerolproducer.Yeast.200522(6):423-30.（SCI，IF=2.5）40.HongXu,WenfangDou,HongyuXu,XiaomeiZhang,ZhimingRao,ZhongpingShi,ZhenghongXu.Atwo-stageoxygensupplystrategyforenhancedL-arginineproductionbyCorynebacteriumcrenatumbasedonmetabolicfluxesanalysis.BiochemicalEngineeringJournal.2009,43:41-51（SCI，IF=2.6）41.Zhuang,JY,Fan,YY,Rao,ZM,Wu,JL,*Xia,YW.Analysisonadditiveeffectsandadditive-by-additiveepistaticeffectsofQTLsforyieldtraitsinarecombinantinbredlinepopulationofrice.TheoreticalandAppliedGenetics,105(8),pp1137-1145,2002/12.（SCI，IF=3.5）42.DouW,XuM,CaiD,ZhangX,RaoZ,*XuZ.Improvementofl-ArginineProductionbyOverexpressionofaBifunctionalOrnithineAcetyltransferaseinCorynebacteriumcrenatum.AppliedBiochemistryandBiotechnology,165(3-4),pp845-855,2011/10.（SCI，IF=2.0）43.ShenW,WangZ,RaoZ,ZhugeJ,ZhugeB.Agenetictransformationsystembasedontrp1complementationinCandidaglycerinogenes.WorldJournalofMicrobiology&Biotechnology,27(4),pp1005-1008,2011/4.（SCI，IF=1.5）44.ChenX,FangH,RaoZ,ShenW,ZhugeB,WangZ,ZhugeJ.ComparativeCharacterizationofGenesEncodingGlycerol3-phosphateDehydrogenaseFromCandidaglycerinogenesandSaccharomycescerevisiae.ProgressinBiochemistryandBiophysics,36(2),pp198-205,2009/2.（SCI）45.Rahman,MuhammadMasfiqur,Qin,ZhaoQin,Dou,Wenfang,RaoZhiming,Xu,Zhenghong*.Over-expressionofNADkinaseinCorynebacteriumcrenatumanditsImpactonL-ArginineBiosynthesis.TropicalJournalofPharmaceuticalResearch,11(6),pp909-916,2012/12.（SCI）46.马正、饶志明*、沈微、方慧英、诸葛健*.一步法产1,3-丙二醇酿酒酵母基因工程菌的构建.微生物学报，2007,47(4):598-60347.丁春生，饶志明*，沈微，诸葛斌，陈献忠，方慧英*，诸葛健.利用绿色荧光蛋白研究产甘油假丝酵母胞浆3-磷酸甘油脱氢酶基因CgGPD启动子的功能.微生物学报，2008，48（8）：1013-101848.刘爱玲,饶志明*,马正,诸葛斌,方慧英,诸葛健*.新一代产甘油重组菌的构建及其初步发酵.生物工程学报.2009,25(6):946-95249.许虹,窦文芳,许泓瑜,张晓梅,饶志明,许正宏.不同供氧水平对l-精氨酸分批发酵过程的影响.化工学报,2008，59(9):2295-230150.陶帅,窦文芳,张晓梅,许泓瑜,饶志明,许正宏.高产精氨酸的钝齿棒杆菌在高低供氧条件下的差异蛋白质组学初步研究.化工学报,2011,(6):1649-1655.51.徐美娟,张显,饶志明*,杨娟,窦文芳,金坚,许正宏*.钝齿棒杆菌N-乙酰鸟氨酸转氨酶的克隆表达分析及其重组菌的精氨酸发酵.生物工程学报,2011,27(7):1013&minus1023.52.田灵芝,徐美娟,饶志明*.一株重组大肠杆菌/pET-28a-lpgad的构建及其高效生产&gamma-氨基丁酸转化条件的优化.生物工程学报,2012,28(1):65~7553.孙红梅,饶志明*,李秀鹏,徐美娟,张显,许正宏.以葡萄糖为底物一步法合成&gamma-氨基丁酸整合型重组钝齿棒杆菌的构建[J].微生物学报.2013,53(8):817-82454.张博慧，徐美娟，饶志明*，许正宏.钝齿棒杆菌溶氧诱导型启动子的筛选及其功能验证.微生物学报，2013,53(9):933-94255.杨套伟，饶志明*，张显，徐美娟，许正宏*.pH与溶氧控制策略对解淀粉芽孢杆菌发酵粗甘油生产2,3丁二醇的影响.生物工程学报.2013,29(12):1860-1864.近5年来主持或完成教育部新世纪优秀人才计划项目1项，国家973计划子课题1项，国家863计划2项、国家自然科学基金3项、江苏省青年科技创新人才基金1项，企业委托基金多项。其中部分科研项目如下:1、教育部新世纪优秀人才项目：微生物高效合成丁二醇代谢工程及其发酵调控（NO.NCET-10-0459）；2、教育部重点科学项目：棒杆菌基因组系统工程改造及其发酵调控3、国家973项目子课题：用合成生物学方法优化微生物基因组（NO.2012CB725202）；4、国家863重大项目子课题：甾醇化合物生物转化关键技术（NO.2011AA02A211）5、国家863重大项目子课题：“工业微生物基因组及分子改造”氨基酸生产菌株发酵过程分析与工艺优化（2012AA022102）6、国家自然科学基金项目“以葡萄糖为底物产PDO酵母代谢工程研究”（NO.**）；7、国家自然科学基金项目“酵母甘油合成关键酶催化分子机理研究”（NO.**）；8、国家自然科学基金项目“解淀粉芽孢杆菌高效合成四碳平台化合物2,3丁二醇机理研究”（NO.**）9、国家青年自然科学基金项目“钝齿棒杆菌精氨酸合成关键酶N-乙酰谷氨酸激酶结构及其反馈抑制调节机制解析（**）”10、江苏省青年自然科学基金“钝齿棒杆菌NAGK抗反馈抑制定点改造及其催化机理解析（BK**）